De novo assembled genomes of the two Flint lines EP1 and F7 were generated using NRGene´s DeNovoMAGIC 2.0. Structural and functional annotation of the two genomes is currently in progress. The two high-quality genomes, Zm-EP1-REFERENCE-TUM-1.0 (Zm00010a) and Zm-F7-REFERENCE-TUM-1.0 (Zm00011a), were released in collaboration with the Maize Genetics and Genomics Database (MaizeGDB; http://www.maizegdb.org) and NCBI (BioProjects PRJNA360920 and PRJNA360923) in accordance with guidelines set forth by the Toronto Agreement for prepublication data sharing (Nature. 2009 461:168).
Unterseer S*, Seidel MA*, Bauer E, Haberer G, Hochholdinger F, Opitz N, Marcon C, Baruch K, Spannagl M, Mayer KFX, Schön C-C (2017) European Flint reference sequences complement the maize pan-genome. bioRxiv, doi: https://doi.org/10.1101/103747. * These authors contributed equally.
Illumina sequence data from 30 diverse Dent and Flint inbred lines from Europe and the US and from one Teosinte accession were generated. The average genome coverage of these 31 lines is 18.9 x. Sequence data are available from the NCBI Sequence Read Archive under BioProject number PRJNA260788 (http://www.ncbi.nlm.nih.gov/bioproject/PRJNA260788). For a description of the plant material see Unterseer et al. 2014.
Unterseer S, Bauer E, Haberer G, Seidel M, Knaak C, Ouzunova M, Meitinger T, Strom TM, Fries R, Pausch H, Bertani C, Davassi A, Mayer KFX, Schön C-C (2014) A powerful tool for genome analysis in maize: development and evaluation of the high density 600 k SNP genotyping array. BMC Genomics 15:823, doi:10.1186/1471-2164-15-823