In total, 1,015 doubled-haploid lines derived from three European flint maize landraces were generated: 516 from Kemater Landmais Gelb (KE), 432 from Petkuser Ferdinand Rot (PE), and 67 from Lalin (LL). The landraces were pre-selected for phenotypic variation in cold-related traits assessed in field trials and population genetic analyses. For a detailed description of this biological resource, see Hölker et al. (2019):
Hölker AC, Mayer M, Presterl T, Bolduan T, Bauer E, Ordas B, Brauner PC, Ouzunova M, Melchinger AE, Schön CC (2019) European maize landraces made accessible for plant breeding and genome-based studies.
Theor Appl Genet 132:3333-3345, doi.org/10.1007/s00122-019-03428-8
Mayer M, Hölker AC, González-Segovia E, Bauer E, Presterl T, Ouzunova M, Melchinger AE, Schön CC (2020) Discovery of beneficial haplotypes for complex traits in maize landraces. Nature Commun 11:4954, doi.org/10.1038/s41467-020-18683-3
Mayer M, Hölker AC, Presterl T, Ouzunova M, Melchinger AE, Schön CC (2022) Genetic diversity of European maize landraces: Dataset on the molecular and phenotypic variation of derived doubled-haploid populations. Data in Brief 42:108164, doi.org/10.1016/j.dib.2022.108164
A rapid cycling genomic selection experiment with three selection cycles was successfully implemented using the DH library described by Hölker et al. 2019 as training set and as starting population. Selection experiment was replicated three times, two times for early developmental traits and one for testcross biomass yield. In total, 9 heterozygous selection populations and 9 DH populations were generated, with 6,937 and 1,215 genotypes, respectively. For detailed description of this biological resource see Polzer et al. (2025) and Rivera-Poulsen et al. (2026):
Polzer C, Auinger HJ, Terán Pineda M, Hölker AC, Mayer M, Presterl T, Rivera Poulsen C, da Silva S, Ouzunova M, Melchinger AE, Schön CC (2025) Rapid cycling genomic selection in maize landraces. Theor Appl Genet 138,75; doi.org/10.1007/s00122-025-04855-6
Rivera-Poulsen C, Polzer C, Hölker AC, Presterl T, da Silva S, Terán-Pineda M, Ouzunova M, Melchinger AE, Schön CC. (2026) Rapid cycling genomic selection in maize landraces – a step towards closing the yield gap. Theor Appl Genet.139, doi.org/10.1007/s00122-025-05107-3
One multi-parental dent MAGIC DH library segregating for drought and heat tolerance was established which comprises 500 DH lines derived from eight non-BSSS inbred lines.
Lin YC, Urbany C, Shlykova A, Hölker A, Ouzunova M, Presterl T, Pook T, Mayer M, Urzinger S, Schön CC. Genetic dissection and correlated proxy traits under suboptimal conditions. Submitted